产品编号：N381268

产品规格：2.5mg

产品价格：面议

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产品详情

标准品编号:	N381268
名称:	C-12 NBD-dihydro-Ceramide
别名:	6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
CAS号:	474943-05-0
分子式:	C₃₆H₆₃N₅O₆
分子量:	661.92
种类:	Research Tools; Molecular Biology; Fluorescent Reagents;
应用:	C-12 NBD-dihydro-Ceramide is a fluorescent ceramidase substrate. C-12 NBD-dihydro-Ceramide compound is a fluorescent ceramide analog which contains a saturated bond in the C4-C5 position of the sphingosine backbone. It can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources. C-12 NBD-dihydro-ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide. This is based on the observation that saturation of the C4-C5 sphingosine bond in C16-ceramide results in approximately a 10-fold reduction in efficiency as a substrate for rat brain ceramidase compared to the unsaturated substrate. Yet, experimental characterization of C-12 NBD-dihydro-ceramide as a ceramidase substrate needs to be performed.
参考文献	1 Tani, M., Okino, N., Mori, K., et al. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem 275(15) 11229-11234 (2000). 2 Ferlinz, K., Kopal, G., Bernardo, K., et al. Human acid ceramidase. Processing, glycosylation, and lysosomal targeting. J Biol Chem 276 (38) 35352-35360 (2001). 3 Tani, M., Okino, N., Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J Biochem 125 746-749 (1999). 4 Bawab, S.E., Usta, J., Roddy, P., et al. Substrate specificty of rat brain ceramidase. J Lipid Res 43 141-148 (2002).