L-Histidine 7-amido-4-methylcoumarin

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产品编号:H456035

产品规格:25mg

产品价格:面议


产品详情

标准品编号: H456035
名称: L-Histidine 7-amido-4-methylcoumarin
别名: (αS)-α-Amino-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-1H-Imidazole-4-propanamide
CAS号: 191723-64-5
分子式: C₁₆H₁₆N₄O₃
分子量: 312.32
种类: Research Tools; Molecular Biology; Fluorescent Reagents;
应用: L-Histidine 7-amido-4-methylcoumarin is an enzyme substrate. Substrates of glycosidases, phosphatases and esterases are commonly employed in microbiology, food/water, environmental and ELISA testing. Assays of peptidases are more important in clinical enzyme assays and AMC is the fluorophore employed in most of these substrates. AMC is extremely versatile since the carboxy-terminus of any amino acid or peptide can be linked to it. Most enzymes are tolerant towards the AMC structure. Typically, kinetics of enzyme reactions remain undisturbed and few inhibitory effects are observed. Due to the low basicity of the amino group, AMC is not subject to acid base equilibrium at physiological pH. Therefore, assays using AMC substrates are rather tolerant of pH.
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